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Human α-thrombin detection platform using aptamers on a silicon nanowire field-effect transistor

: Römhildt, Lotta; Zörgiebel, Felix; Ibarlucea, Bergoi; Vahdatzadeh, Maryam; Baraban, Larysa; Cuniberti, Gianaurelio; Pregl, Sebastian; Weber, Walter M.; Mikolajick, Thomas; Opitz, Jörg


Institute of Electrical and Electronics Engineers -IEEE-:
27th International Symposium on Power and Timing Modeling, Optimization and Simulation, PATMOS 2017 : 25-27 September 2017, Thessaloniki, Greece
Piscataway, NJ: IEEE, 2017
ISBN: 978-1-5090-6463-2
ISBN: 978-1-5090-6462-5
ISBN: 978-1-5090-6461-8
4 pp.
International Symposium on Power and Timing Modeling, Optimization and Simulation (PATMOS) <27, 2017, Thessaloniki>
Conference Paper
Fraunhofer IKTS ()
silicon nanowire; field effect transistor; aptasensors; aptamers; human α-thrombin

We present a silicon nanowire-based field-effect transistor biosensor with Schottky barriers for highly specific and sensitive human α-thrombin detection. The active sensor area is decorated with thrombin-binding aptamers as receptor molecules. Each sensor chip is integrated into a microfluidic device for flow-through measurements. Instantaneous detection is provided by real-time monitoring of FET transfer curves. With this approach, thrombin concentrations between 200 pM and 200 nM are detected in a label-free, real-time manner, covering a wide dynamic range and enabling to distinguish between healthy and pathological levels. The development of simple, miniaturized devices for blood protein detection is highly interesting for medical diagnostics.