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2018
Journal Article
Titel
Biocatalytically active thin films via self-assembly of 2-deoxy-D-ribose-5-phosphate aldolase-Poly(N-isopropylacrylamide) conjugates
Alternative
Biocatalytically active thin films via self-assembly of 2-deoxy-D-ribose-5-phosphate aldolase/PNIPAm conjugates
Abstract
2-Deoxy-D-ribose-5-phosphate aldolase (DERA) is a biocatalyst that is capable of converting acetaldehyde and a second aldehyde as acceptor into enantiomerically pure mono- and diyhydroxyaldehydes, which are important structural motifs in a number of pharmaceutically active compounds. However, substrate as well as product inhibition requires more a sophisticated process design for the synthesis of these motifs. One way to do so is to couple aldehyde conversion with transport processes, which in turn would require an immobilization of the enzyme within a thin film that can be deposited on a membrane support. Consequently, we developed a fabrication process for such films, which is based on the formation of DERA/poly(N-isopropyl acrylamide) conjugates that are subsequently allowed to self-assemble at an air/water interface to yield the respective film. In this contribution, we discuss the conjugation conditions, investigate the interfacial properties of the conjugates and finally demonstrate a successful film formation under preservation of enzymatic activity.
Author(s)
Bisterfeld, Carolin
Institut für Bioorganische Chemie, Heinrich-Heine-Universität Du&sseldorf am Forschungszentrum Ju&lich, Ju&lich
Bramski, Julia
Institut für Bioorganische Chemie, Heinrich-Heine-Universität Du&sseldorf am Forschungszentrum Ju&lich, Ju&lich
Pietruszka, Jörg
Institut für Bioorganische Chemie, Heinrich-Heine-Universität Du&sseldorf am Forschungszentrum Ju&lich, Ju&lich und IBG-1: Biotechnologie, Forschungszentrum Ju&lich GmbH, Ju&lich