
Publica
Hier finden Sie wissenschaftliche Publikationen aus den Fraunhofer-Instituten. Labbag® - a versatile bag-based cultivation system for expansion, differentiation and cryopreservation of human stem cells
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Gepp, Michael; Duckstein, Rowena; Kayatz, Fabian; Rodler, Norbert; Scheuerer, Zuzana; Neubauer, Julia; Lachmann, Kristina; Stramm, Cornelia; Liebmann, Andrea; Thomas, Michael; Zimmermann, Heiko :
Fulltext urn:nbn:de:0011-n-4743699 (490 KByte PDF) MD5 Fingerprint: 16c7a5852d3bfda40928e55dc1226542 Created on: 5.12.2017 |
Abstract
Novel approaches in regenerative medicine and tissue engineering are highly promoted by human induced pluripotent stem cells. Typical workflows include the expansion, differentiation and/or cryopreservation of these cells. Often, the differentiation, cytotoxicity tests or disease modelling rely on multicellular aggregates. Here, we present the novel bag-based system “Labbag®” to address workflows required for these applications. As a unique feature, the Labbag® provides a chemical spot pattern to allow the formation of several hundreds of hanging droplets within a few seconds with standard laboratory equipment. In this study, we describe the general concept of the Labbag® and show aggregate formation of stem cells as first stem cell workflow successfully transferred.