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SNAP-tag technology. A promising tool for ex vivo immunophenotyping

: Choudhary, S.; Barth, S.; Verma, R.S.


Molecular diagnosis & therapy 21 (2017), No.3, pp.315-326
ISSN: 1177-1062 (Print)
ISSN: 1179-2000 (Online)
Journal Article
Fraunhofer IME ()

Background: SNAP-tag, a self-labeling protein tag, is commonly used for in vitro and in vivo analysis of bound target proteins. We report the first evidence that SNAP-tag could be used for ex vivo detection of enriched biological markers.
Methods: Proof of concept was established for target c-kit receptor, a pathological and diagnostic marker for a variety of cancers. SNAP-tag conjugates with stem-cell factor (SCF) fusion proteins were designed and their binding and specificity was validated in vitro using flow cytometry and immunostaining.
Results: Ex vivo diagnostic application of the fusion protein was demonstrated in comparison with anti-c-kit antibody for peripheral blood samples from leukemia patients and colorectal tissue specimens.