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In vitro transcription of a whole gene on a surface-coupled template

: Steffen, J.; Nickisch-Rosenegk, M. von; Bier, F.F.


LAB on a chip (2005), No.5, pp.665-668
ISSN: 1473-0197
ISSN: 1473-0189
Journal Article
Fraunhofer IBMT ()

An artificial gene was constructed combining the T7 promoter and terminator with the EGFP-gene from the plasmid pEGFP. The functionality of the construct was shown by in vitro translation. The gene-construct was immobilised on a planar glass surface. The transcription was performed on the immobilised gene and mRNA was determined by RT-PCR. Multiple use of the immobilised gene was demonstrated.