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An integrated versatile lab-on-a-chip platform for the isolation and nucleic acid-based detection of pathogens

 
: Sandetskaya, Natalia; Moos, Doreen; Pötter, Harald; Seifert, Stefan; Jenerowicz, Marcin; Becker, Holger; Zilch, Christian; Kuhlmeier, Dirk

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Future Science OA 3 (2017), No.2, Art. FSO177, 13 pp.
ISSN: 2056-5623
English
Journal Article, Electronic Publication
Fraunhofer IZI ()
Fraunhofer IZM ()

Abstract
Aim: Processing of the samples in molecular diagnostics is complex and labor-intensive. An integrated and automated platform for sample preparation and nucleic acid-based detection can significantly relieve this burden for the users. Results: We present a prototype of a versatile and integrated platform for the detection of pathogens in various liquid media. We describe a proof-of-concept for the integrated isolation of bacteria, cell lysis with optional DNA extraction, DNA amplification and detection in two different reactions, loop-mediated isothermal amplification and PCR, on a single microfluidic platform. Conclusion: The platform enables the transition from large sample volume to microfluidic format. The design and open interface enable its versatile application for various nucleic acid-based assays, from simple to complex setups.

: http://publica.fraunhofer.de/documents/N-456250.html