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Intubation-free in vivo imaging of the tracheal mucosa using two-photon microscopy

: Veres, Tibor Z.; Kopcsányi, Támas; Tirri, Marko; Braun, Armin; Miyasaka, Masayuki; Germain, Ronald N.; Jalkanen, Sirpa; Salmi, Marko

Fulltext urn:nbn:de:0011-n-4524532 (3.1 MByte PDF)
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Created on: 14.6.2017

Scientific Reports 7 (2017), Art. 694, 9 pp.
ISSN: 2045-2322
Journal Article, Electronic Publication
Fraunhofer ITEM ()
experimental models of disease; multiphoton microscopy; time-lapse imaging

The mucosal layer of conducting airways is the primary tissue exposed to inhaled microorganisms, allergens and pollutants. We developed an in vivo two-photon microscopic approach that allows performing dynamic imaging studies in the mouse trachea, which is a commonly used in vivo model of human small-diameter bronchi. By providing stabilized access to the tracheal mucosa without intubation, our setup uniquely allows dynamic in vivo imaging of mucociliary clearance and steady-state immune cell behavior within the complex airway mucosal tissue.