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Title
Metallbindende, proteinhaltige Verbindungen, Verfahren zu deren Gewinnung und ihre Verwendung
Date Issued
2002
Author(s)
Patent No
2000-10050873
Abstract
Die Erfindung betrifft neue metallbindende Verbindungen, die aus Getreidekeimlingen isoliert wurden und mit hoher Affinitaet Schwermetallionen binden. Gegenstand der Erfindung ist auch das Verfahren zur Gewinnung dieser Verbindungen sowie deren Verwendung und die Verwendung des Rohextraktes von Getreidekeimlingen als Metallabsorber.
DE 10050873 A UPAB: 20020722 NOVELTY - Metal-binding proteins (I) isolated from cereal seedling extracts are new. DETAILED DESCRIPTION - Metal-binding proteins (I) that form copper (Cu2+), cadmium (Cd2+) or zinc (Zn2+) complexes with a molecular weight of 25-30 kD and have a N-terminal sequence with at least 90% homology to one of the following sequences are new: Xaa Gly Pro Gly Met Pro Tyr Xaa Xaa Gln Met (seq 1); Xaa Gly Pro Gly Met Pro Tyr Xaa Xaa Gln Leu (seq 2); Xaa Pro Pro Gly Met Pro Tyr Xaa Xaa Gln Met (seq 3); Xaa Pro Pro Gly Met Pro Tyr Xaa Xaa Gln Leu (seq 4); Xaa Ala Glu Val Ser Xaa Ala Ala Gln Leu Xaa Thr Ala Xaa (seq 5). INDEPENDENT CLAIMS are also included for the following: (1) producing (I), by either: (a) incubating cereal grains with an aqueous heavy metal solution, germinating the grains, harvesting the roots from the seedlings, disrupting the roots, and isolating (I) from the resulting crude root extract by conventional protein separation methods; or (b) germinating cereal seedlings in tap water, disrupting the seedlings, chromatographing the resulting crude extract on a separation medium capable of reversibly binding metals, eluting bound substances, and isolating (I) from the eluate by conventional protein separation methods; (2) use of a crude cereal seedling extract for binding heavy metal ions, where the extract is produced by disrupting cereal seedlings germinated in tap water; (3) use of a crude cereal seedling root extract for binding heavy metal ions, where the extract is produced by incubating cereal grains with an aqueous heavy metal solution, germinating the grains, harvesting the roots from the seedlings and disrupting the roots. USE - (I) are useful, preferably in immobilized form, for removing and recovering heavy metals from fluid media.
Language
de
Patenprio
DE 2000-10050873 A: 20001011