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Quantitative bioimaging of platinum group elements in tumor spheroids

: Niehoff, A.-C.; Grünebaum, J.; Moosmann, A.; Mulac, D.; Söbbing, J.; Niehaus, R.; Buchholz, R.; Kröger, S.; Wiehe, A.; Wagner, S.; Sperling, M.; Briesen, H. von; Langer, K.; Karst, U.


Analytica Chimica Acta 938 (2016), pp.106-113
ISSN: 0003-2670
ISSN: 0378-4304
Journal Article
Fraunhofer IBMT ()

Limited drug penetration into tumor tissue is a significant factor to the effectiveness of cancer therapy. Tumor spheroids, a 3D cell culture model system, can be used to study drug penetration for pharmaceutical development. In this study, a method for quantitative bioimaging of platinum group elements by laser ablation (LA) coupled to inductively coupled plasma mass spectrometry (ICP-MS) is presented. Different matrix-matched standards were used to develop a quantitative LA-ICP-MS method with high spatial resolution. To investigate drug penetration, tumor spheroids were incubated with platinum complexes (Pt(II)acetylacetonate, cisplatin) and the palladium tagged photosensitizer 5,10,15,20-tetrakis(3-hydroxyphenyl)porphyrin (mTHPP). Distribution and accumulation of the pharmaceuticals were determined with the developed method.