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Title
Verfahren zur Isolierung von Erythrozyten
Date Issued
2000
Author(s)
Patent No
1999-19912120
Abstract
Die Erfindung betrifft ein verbessertes Verfahren zur Isolierung interessierender Bestandteile aus biologischen Proben. Dabei werden die interessierenden Bestandteile an Makromolekuelen reversibel gebunden.
DE 19912120 A UPAB: 20001117 NOVELTY - Isolation of erythrocytes (E) comprising treating a sample with at least one substance (I) that promotes aggregation of (E), and separating the aggregates, is new. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is also included for a device for isolation of (E) comprising at least one (I) bound to a carrier. USE - The method is used to eliminate (E) from samples before polymerase chain reaction (where (E) act as inhibitors of chain extension), and to recover (E) for subsequent analysis. ADVANTAGE - The method is simpler and less expensive than conventional methods that require centrifugation or specific antibodies. No non-specific binding of leukocytes, viruses and bacteria to (I) occurs, so these do not become incorporated into the aggregates. (E) are reversibly bound to (I) and do not undergo hemolysis.
Language
de
Patenprio
DE 1999-19912120 A: 19990318