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Verfahren zur Isolierung von Erythrozyten

Isolating erythrocytes, useful e.g. for pretreatment of samples before polymerase chain reaction, by treatment with agent that promotes aggregation.
 
: Brunner, H.; Walitza, E.; Geray, J.; Vitzthum, F.; Tovar, G.

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Frontpage ()

DE 1999-19912120 A: 19990318
DE 1999-19912120 A: 19990318
WO 2000-EP2352 A: 20000316
DE 19912120 A1: 20000928
C12N0005
G01N0033
German
Patent, Electronic Publication
Fraunhofer IGB ()

Abstract
Die Erfindung betrifft ein verbessertes Verfahren zur Isolierung interessierender Bestandteile aus biologischen Proben. Dabei werden die interessierenden Bestandteile an Makromolekuelen reversibel gebunden.

 

DE 19912120 A UPAB: 20001117 NOVELTY - Isolation of erythrocytes (E) comprising treating a sample with at least one substance (I) that promotes aggregation of (E), and separating the aggregates, is new. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is also included for a device for isolation of (E) comprising at least one (I) bound to a carrier. USE - The method is used to eliminate (E) from samples before polymerase chain reaction (where (E) act as inhibitors of chain extension), and to recover (E) for subsequent analysis. ADVANTAGE - The method is simpler and less expensive than conventional methods that require centrifugation or specific antibodies. No non-specific binding of leukocytes, viruses and bacteria to (I) occurs, so these do not become incorporated into the aggregates. (E) are reversibly bound to (I) and do not undergo hemolysis.

: http://publica.fraunhofer.de/documents/N-44421.html