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  4. Lab-on-a-Chip multiplex assays
 
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2017
Book Article
Title

Lab-on-a-Chip multiplex assays

Abstract
Lab-on-a-chip multiplex assays allow a rapid identification of multiple parameters in an automated manner. Here we describe a lab-based preparation followed by a rapid and fully automated DNA microarray hybridization and readout in less than 10 min using the Fraunhofer in vitro diagnostics (ivD) platform to enable rapid identification of bacterial species and detection of antibiotic resistance. The use of DNA microarrays allows a fast adaptation of new biomarkers enabling the identification of different genes as well as single-nucleotide-polymorphisms (SNPs) within these genes. In this protocol we describe a DNA microarray developed for identification of Staphylococcus aureus and the mecA resistance gene.
Author(s)
Peter, Harald
Fraunhofer IZI-BB
Wienke, Julia
Fraunhofer IZI-BB
Bier, Frank F.
Fraunhofer IZI-BB
Mainwork
Multiplex biomarker techniques  
DOI
10.1007/978-1-4939-6730-8_25
Language
English
Fraunhofer-Institut für Zelltherapie und Immunologie IZI  
Keyword(s)
  • bacterical infection

  • antimicrobial drug resistance

  • antibiotic resistance detection

  • MRSA detection

  • genotyping

  • SNP detection

  • automated DNA microarray hybridization

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