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Plant Cell-Based Recombinant Antibody Manufacturing with a 200 L Orbitally Shaken Disposable Bioreactor

: Raven, N.; Schillberg, S.; Rasche, S.


Recombinant Proteins from Plants : Methods and Protocols 1385 (2016), pp.161-172
ISBN: 978-1-4939-3289-4 (online)
ISBN: 978-1-4939-3288-7 (print)
URN: urn:nbn:de:1111-20151128132
DOI: 10.1007/978-1-4939-3289-4
Book Article
Fraunhofer IME ()

Tobacco BY-2 cells are an attractive platform for the manufacture of a variety of biopharmaceutical proteins, including antibodies. Here, we describe the scaled-up cultivation of human IgG–secreting BY-2 cells in a 200 L orbitally shaken disposable bioreactor, resulting in cell growth and recombinant protein yields that are proportionately comparable with those obtained from cultivations in 500 mL shake flasks. Furthermore, we present an efficient downstream process for antibody recovery from the viscous spent culture medium using expanded bed adsorption (EBA) chromatography.