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Microfluidic system for in-vitro hypoxia assays

: Busek, Mathias; Grünzner, Stefan; Steege, Tobias; Steinfelder, C.; Schmieder, Florian; Klotzbach, Udo; Sonntag, Frank

Fulltext urn:nbn:de:0011-n-4359136 (1.3 MByte PDF)
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Created on: 30.5.2017

Gray, B.L. ; Society of Photo-Optical Instrumentation Engineers -SPIE-, Bellingham/Wash.:
Microfluidics, BioMEMS, and Medical Microsystems XV : San Francisco, California, United States, 28 - 30 January 2017
Bellingham, WA: SPIE, 2017 (Proceedings of SPIE 10061)
Paper 1006110, 10 pp.
Conference "Microfluidics, BioMEMS, and Medical Microsystems" <15, 2017, San Francisco/Calif.>
Bundesministerium für Wirtschaft und Technologie BMWi
Conference Paper, Electronic Publication
Fraunhofer IWS ()
hypoxia; cell culture; Lab-on-a-Chip; Perfusion; μPIV; Microfluidics

Hereby presented is a microfluidic system, including a micro pump, an oxygenator and a cell culture chamber for perfusion controlled hypoxia assays. It consists of laser-structured polycarbonate (PC) foils and an elastomeric membrane which were joined together using thermal diffusion bonding. The elastomer forms an oxygenator element. The microfluidic system is characterized using non-invasive flow measurement based on micro-Particle-Image- Velocimetry (μPIV) and optical oxygen measurement utilizing the oxygen dependent fluorescence decay. Based on those experimental results and mathematical considerations, the oxygenator and mass transport phenomena within the microfluidic system can be described. This oxygen sensor, the micro pump, a controlling device and the gas mixture at the oxygenator forms a regulatory circuit to adjust the oxygen content in the cell culture chamber and helps to produce well-defined hypoxic conditions for the cells.