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The molecular blueprint of a fungus by next-generation sequencing (NGS)

 
: Grumaz, Christian; Kirstahler, Philipp; Sohn, Kai

:

Lion, T.:
Human fungal pathogen identification. Methods and protocols
New York/NY: Humana Press, 2017 (Methods in molecular biology 1508)
ISBN: 978-1-4939-6513-7 (Print)
ISBN: 978-1-4939-6515-1 (Online)
ISBN: 1-4939-6513-1
pp.361-383
English
Book Article
Fraunhofer IGB ()
next-generation sequencing (NGS); whole-genome shotgun sequencing (WGS); RNA sequencing (RNA-seq); Fungal genome; assembly; annotation; Bioinformatics

Abstract
Sequencing the whole genome of an organism is invaluable for its comprehensive molecular characterization and has been drastically facilitated by the advent of high-throughput sequencing techniques. Especially in clinical microbiology the impact of sequenced strains increases as resistance and virulence markers can easily be detected. Here, we describe a combined approach for sequencing a fungal genome and transcriptome from initial nucleic acid isolation through the generation of ready-to-load DNA libraries for the Illumina platform and the final step of genome assembly with subsequent gene annotation.

: http://publica.fraunhofer.de/documents/N-435719.html