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Encapsulation of FRET-based glucose and maltose biosensors to develop functionalized silica nanoparticles

 
: Faccio, Greta; Bannwarth, Markus B.; Schulenburg, Cindy; Steffen, V.; Jankowska, Dagmara A.; Pohl, M.; Rossi, René; Maniura-Weber, Katharina; Boesel, Luciano; Richter, Martin

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Analyst 141 (2016), No.13, pp.3982-3984
ISSN: 0003-2654
ISSN: 1364-5528
English
Journal Article
Fraunhofer IGB ()

Abstract
Silicate nanoparticles with immobilized FRET-based biosensors were developed for the detection of glucose and maltose. Immobilization of the protein biosensor in the nanoparticle was achieved through specific interaction between the hexa-histidine tag of the protein and a calcium–silicate complex of the silica matrix. Encapsulation of the biosensors preserved the affinity for the respective sugar. Compared to the free biosensors, encapsulation had a stabilizing effect on the biosensor towards chemical and thermal denaturation. The demonstrated immobilization strategy for specific sensing proteins paves the way towards the development of protein-inorganic nanostructures for application in metabolite analyses.

: http://publica.fraunhofer.de/documents/N-435677.html