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Functional and structural characterisation of a bacterial O-methyltransferase and factors determining regioselectivity

: Siegrist, J.; Netzer, J.; Mordhorst, S.; Karst, L.; Gerhardt, S.; Einsle, O.; Richter, M.; Andexer, J.N.


FEBS Letters 591 (2017), No.2, pp.312-321
ISSN: 0014-5793
ISSN: 1873-3468
Journal Article
Fraunhofer IGB ()

Mg2+-dependent catechol-O-methyltransferases occur in animals as well as in bacteria, fungi and plants, often with a pronounced selectivity towards one of the substrate's hydroxyl groups. Here, we show that the bacterial MxSafC exhibits excellent regioselectivity for para as well as for meta methylation, depending on the substrate's characteristics. The crystal structure of MxSafC was solved in apo and in holo form. The structure complexed with a full set of substrates clearly illustrates the plasticity of the active site region. The awareness that a wide range of factors influences the regioselectivity will aid the further development of catechol-O-methyltransferases as well as other methyltransferases as selective and efficient biocatalysts for chemical synthesis.