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Organ-like three dimensional test systems

: Rossi, Angela; Groeber, Florian; Steinke, Maria; Dandekar, Gudrun; Nietzer, Sarah; Metzger, Marco; Walles, Heike

ALTEX Proceedings 3 (2014), No.1, pp.224, Abstract VI-4b-313
ISSN: 2194-0479
World Congress on Alternatives and Animal Use in the Life Sciences <9, 2014, Prag>
Fraunhofer IGB ()
3D model; Test-System; BioVaSc®; skin model; intestine; tumor model

Products must be tested for their quality, safety, and efficacy. Two dimensional models (2D) have been widely used to analyze physiological effects. However, conclusions are limited and less obvious compared to the in-vivo situation. Three dimensional (3D) cell cultures possess many features that mimic the in-vivo microenvironment which cannot be detected in the traditional 2D cell cultures. Therefore, we developed alternative human test systems that reflect the complex characteristics of the body. We work with three different skin models: an epidermal model, a full skin model and a vascularized skin model, which can be extended by other cells (melanocytes, skin tumor cells). The models are suitable for measuring penetration, distribution, and metabolization of test substances. Additionally, effects concerning the proliferation, differentiation and cell death can be examined. Our group has succeeded in building up both a trachea and an intestine test system based on primary cells and a matrix with a blood vessel system (BioVaSc®; biological vascularized scaffold). We also work with different tumor model systems (intestine, breast, lung, Neurofibroma, leukemia) based on the BioVaSc®. To ensure the functionality of these cells in vitro, models are cultured in specific bioreactor systems with conditions similar to the natural microenvironment.