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Patterns of CRISPR/Cas9 activity in plants, animals and microbes

: Bortesi, L.; Zhu, C.; Zischewski, J.; Perez, L.; Bassié, L.; Nadi, R.; Forni, G.; Lade, S.B.; Soto, E.; Jin, X.; Medina, V.; Villorbina, G.; Munoz, P.; Farré, G.; Fischer, R.; Twyman, R.M.; Capell, T.; Christou, P.; Schillberg, S.


Plant biotechnology journal 14 (2016), No.12, pp.2203-2216
ISSN: 1467-7644
Journal Article
Fraunhofer IME ()

The CRISPR/Cas9 system and related RNA-guided endonucleases can introduce double-strand breaks (DSBs) at specific sites in the genome, allowing the generation of targeted mutations in one or more genes as well as more complex genomic rearrangements. Modifications of the canonical CRISPR/Cas9 system from Streptococcus pyogenes and the introduction of related systems from other bacteria have increased the diversity of genomic sites that can be targeted, providing greater control over the resolution of DSBs, the targeting efficiency (frequency of on-target mutations), the targeting accuracy (likelihood of off-target mutations) and the type of mutations that are induced. Although much is now known about the principles of CRISPR/Cas9 genome editing, the likelihood of different outcomes is species-dependent and there have been few comparative studies looking at the basis of such diversity.