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Mice produced by mitotic reprogramming of sperm injected into haploid parthenogenotes

 
: Suzuki, Toru; Asami, Maki; Hoffmann, Martin; Lu, Xin; Guzvic, Miodrag; Klein, Christoph A.; Perry, Anthony C.F.

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Fulltext urn:nbn:de:0011-n-4216921 (3.6 MByte PDF)
MD5 Fingerprint: be5a795125991172ed81d99b854ead70
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Created on: 4.1.2017


Nature Communications 7 (2016), Art. 12676, 15 pp.
ISSN: 2041-1723
English
Journal Article, Electronic Publication
Fraunhofer ITEM ()

Abstract
Sperm are highly differentiated and the activities that reprogram them for embryonic development during fertilization have historically been considered unique to the oocyte. We here challenge this view and demonstrate that mouse embryos in the mitotic cell cycle can also directly reprogram sperm for full-term development. Developmentally incompetent haploid embryos (parthenogenotes) injected with sperm developed to produce healthy offspring at up to 24% of control rates, depending when in the embryonic cell cycle injection took place. This implies that most of the first embryonic cell cycle can be bypassed in sperm genome reprogramming for full development. Remodelling of histones and genomic 5′-methylcytosine and 5′-hydroxymethylcytosine following embryo injection were distinct from remodelling in fertilization and the resulting 2-cell embryos consistently possessed abnormal transcriptomes. These studies demonstrate plasticity in the reprogramming of terminally differentiated sperm nuclei and suggest that different epigenetic pathways or kinetics can establish totipotency.

: http://publica.fraunhofer.de/documents/N-421692.html