Hier finden Sie wissenschaftliche Publikationen aus den Fraunhofer-Instituten.

Bacillus anthracis spore movement does not require a carrier cell and is not affected by lethal toxin in human lung models

: Booth, J.L.; Duggan, E.S.; Patel, V.I.; Langer, M.; Wu, W.; Braun, A.; Coggeshall, K.M.; Metcalf, J.P.


Microbes and infection 18 (2016), No.10, pp.615-626
ISSN: 1286-4579
Journal Article
Fraunhofer ITEM ()

The lung is the entry site for Bacillus anthracis in inhalation anthrax, the most deadly form of the disease. Spores escape from the alveolus to regional lymph nodes, germinate and enter the circulatory system to cause disease. The roles of carrier cells and the effects of B. anthracis toxins in this process are unclear. We used a human lung organ culture model to measure spore uptake by antigen presenting cells (APC) and alveolar epithelial cells (AEC), spore partitioning between these cells, and the effects of B. anthracis lethal toxin and protective antigen. We repeated the study in a human A549 alveolar epithelial cell model. Most spores remained unassociated with cells, but the majority of cell-associated spores were in AEC, not in APC. Spore movement was not dependent on internalization, although the location of internalized spores changed in both cell types. Spores also internalized in a non-uniform pattern.