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High-throughput analysis of global DNA methylation using methyl-sensitive digestion

 
: Shiratori, H.; Feinweber, C.; Knothe, C.; Lötsch, J.; Thomas, D.; Geisslinger, G.; Parnham, M.J.; Resch, E.

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PLoS one. Online journal 11 (2016), No.10, Art. e0163184, 16 pp.
http://www.pubmedcentral.nih.gov/tocrender.fcgi?action=archive&journal=440
ISSN: 1932-6203
English
Journal Article, Electronic Publication
Fraunhofer IME ()

Abstract
DNA methylation is a major regulatory process of gene transcription, and aberrant DNA methylation is associated with various diseases including cancer. Many compounds have been reported to modify DNA methylation states. Despite increasing interest in the clinical application of drugs with epigenetic effects, and the use of diagnostic markers for genomewide hypomethylation in cancer, large-scale screening systems to measure the effects of drugs on DNA methylation are limited. In this study, we improved the previously established fluorescence polarization-based global DNA methylation assay so that it is more suitable for application to human genomic DNA. Our methyl-sensitive fluorescence polarization (MSFP) assay was highly repeatable (inter-assay coefficient of variation = 1.5%) and accurate (r2 = 0.99). According to signal linearity, only 50-80 ng human genomic DNA per reaction was necessary for the 384-well format.

: http://publica.fraunhofer.de/documents/N-421652.html