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Detection of miRNA using a surface plasmon resonance biosensor and antibody amplification

 
: Schmieder, Stefan; Weißpflog, Janek; Danz, Norbert; Klotzbach, Udo; Sonntag, Frank

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Current directions in biomedical engineering 2 (2016), No.1, pp.135-138
ISSN: 2364-5504
Bundesministerium für Bildung und Forschung BMBF
13N12848; DEMOST
English
Journal Article, Electronic Publication
Fraunhofer IWS ()
Fraunhofer IOF ()
Biochip; Biosensor; MiRNA; molecular diagnostic; surface plasmon resonance (SPR)

Abstract
MiRNAs are non-coding RNA molecules that control biological functions by reducing the translation of target proteins when binding to the mRNA. Alterations of the miRNA expression profile affect the cell metabolism, which can lead to distinctive disease patterns thus suggesting miRNA as an interesting biomarker. Here we present a SPR biosensor that utilizes disposable, injection-molded sensor chip/microfluidic hybrids combined with a lateral imaging optical system for parallel analysis of three one-dimensional spot arrays to detect miRNA-93. Using a RNA-DNA-hybrid antibody for signal enhancement we could reach a limit of detection of 10 pmol/l.

: http://publica.fraunhofer.de/documents/N-417412.html