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Stem cell labeling with iron oxide nanoparticles: Impact of 3D culture on cell labeling maintenance

: Kilian, Teresa; Fidler, Florian; Kasten, Annika; Nietzer, Sarah; Landgraf, Veronika; Weiß, Katrin; Walles, Heike; Westphal, Fritz; Hackenberg, Stephan; Grüttner, Cordula; Steinke, Maria


Nanomedicine 11 (2016), No.15, pp.1957-1970
ISSN: 1743-5889
ISSN: 1748-6963
Journal Article
Fraunhofer IGB ()
metal nanoparticles; regenerative medicine; stem cells

Aim: We aimed to analyze the suitability of nanoparticles (M4E) for safe human mesenchymal stem cell (hMSC) labeling and determined cell labeling maintenance in 2D and 3D culture.
Materials & methods: We investigated cell–particle interaction and the particles’ impact on cell viability, growth and proliferation. We analyzed cell labeling maintenance in 2D and 3D culture invasively and noninvasively.
Results: M4E do not affect cell viability, growth and proliferation and do not cause chromosomal aberrations. Cell labeling maintenance is up to five-times higher in 3D conditions compared with 2D culture.
Conclusion: M4E allow safe hMSC labeling and noninvasive identification. Our hMSC-loaded, 3D tissue-engineered construct could serve as a graft for regenerative therapies, in which M4E-labeled hMSCs can migrate to their target.