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pH-driven self-assembly of acidic microbial glycolipids

 
: Baccile, Niki; Selmane, Mohamed; Griel, Patrick le; Prévost, Sylvain; Perez, Javier; Stevens, Christian V.; Delbeke, Elisabeth; Zibek, Susanne; Guenther, Michael; Soetaert, Wim; Bogaert, Inge N.A. van; Roelants, Sophie

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Langmuir. The ACS journal of surfaces and colloids 32 (2016), No.25, pp.6343-6359
ISSN: 0743-7463
ISSN: 1520-5827
English
Journal Article
Fraunhofer IGB ()

Abstract
Microbial glycolipids are a class of well-known compounds, but their self-assembly behavior is still not well understood. While the free carboxylic acid end group makes some of them interesting stimuli-responsive compounds, the sugar hydrophilic group and the nature of the fatty acid chain make the understanding of their self-assembly behavior in water not easy and highly unpredictable. Using cryo-transmission electron microscopy (cryo-TEM) and both pH-dependent in situ and ex situ small angle X-ray scattering (SAXS), we demonstrate that the aqueous self-assembly at room temperature (RT) of a family of β-d-glucose microbial glycolipids bearing a saturated and monounsaturated C18 fatty acid chain cannot be explained on the simple basis of the well-known packing parameter. Using the “pH-jump” process, we find that the molecules bearing a monosaturated fatty acid forms vesicles below pH 6.2, as expected, but the derivative with a saturated fatty acid forms infinite bilayer sheets below pH 7.8, instead of vesicles. We show that this behavior can be explained on the different bilayer membrane elasticity as a function of temperature. Membranes are either flexible or stiff for experiments performed at a temperature respectively above or below the typical melting point, TM, of the lipidic part of each compound. Finally, we also show that the disaccharide-containing acidic cellobioselipid forms a majority of chiral fibers, instead of the expected micelles.

: http://publica.fraunhofer.de/documents/N-415235.html