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2016
Journal Article
Titel
Optimizing the design of a reproduction toxicity test with the pond snail Lymnaea stagnalis
Abstract
This paper presents the results from two ring-tests addressing the feasibility, robustness and reproducibility of a reproduction toxicity test with the freshwater gastropod Lymnaea stagnalis (RENILYS strain). Sixteen laboratories (from inexperienced to expert laboratories in mollusc testing) from nine countries participated in these ring-tests. Survival and reproduction were evaluated in L. stagnalis exposed to cadmium, tributyltin, prochloraz and trenbolone according to an OECD draft Test Guideline. In total, 49 datasets were analysed to assess the practicability of the proposed experimental protocol, and to estimate the between-laboratory reproducibility of toxicity endpoint values. The statistical analysis of count data (number of clutches or eggs per individual-day) leading to ECx estimation was specifically developed and automated through a free web-interface. Based on a complementary statistical analysis, the optimal test duration was established and the most sensitive and cost-effective reproduction toxicity endpoint was identified, to be used as the core endpoint. This validation process and the resulting optimized protocol were used to consolidate the OECD Test Guideline for the evaluation of reproductive effects of chemicals in L. stagnalis.
Author(s)
Charles, Sandrine
Université Lyon - Laboratoire de Biométrie et Biologie Évolutive - Villeurbanne - France
Ducrot, Virginie
Institut National de la Recherche Agronomique (INRA), Centre de Recherche de Rennes - Rennes - France
Azam, Didier
Institut National de la Recherche Agronomique (INRA), Centre de Recherche de Rennes - Rennes - France
Benstead, Rachel
The Food and Environment Research Agency (now Fera Science Ltd), Sand Hutton - York - United Kingdom
Brettschneider, Denise
Goethe University Frankfurt am Main, Department Aquatic Ecotoxicology - Frankfurt - Germany
Schamphelaere, Karel de
Ghent University, Environmental Toxicology Unit - GhEnToxLab - Ghent - Belgium
Goncalves, Sandra Filipe
Department of Biology & CESAM, Centre for Environmental and Marine Studies, University of Aveiro - Aveiro - Portugal
Hutchinson, Thomas H.
School of Biological Sciences, University of Plymouth - Plymouth - United Kingdom
Faber, Daniel
Bayer Aktiengesellschaft, Crop Science Division, BCS AG-R&D-D-EnSa-ETX-AQ - Monheim am Rhein - Germany
Norrgren, Leif
Department of Pathology, Faculty of Veterinary Science, Swedish University of Agricultural Sciences - Uppsala - Sweden
Oehlmann, Jörg
Goethe University Frankfurt am Main, Department Aquatic Ecotoxicology - Frankfurt - Germany
Reategui-Zirena, Evelyn
Department of Environmental Toxicology, Texas Tech University - Lubbock - TX - USA
Thome, Jean-Pierre
University of Liège, Laboratory of Animal Ecology and Ecotoxicity (LEAE-CART) - Sart-Tilman - Belgium
Tobor Kaplon, Marysia
WIL Research, Department of In vitro and Environmental Toxicology - Ashland - United States