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Detecting carbohydrate-lectin interactions using a fluorescent probe based on DBD dyes

: Bader, D.; Klier, D.T.; Hettrich, C.; Bier, F.F.; Wessig, P.

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Analytical methods 8 (2016), No.6, pp.1235-1238
ISSN: 1759-9660
ISSN: 1759-9679
Journal Article, Electronic Publication
Fraunhofer IZI ()

Herein we present an efficient synthesis of a biomimetic probe with modular construction that can be specifically bound by the mannose binding FimH protein - a surface adhesion protein of E. coli bacteria. The synthesis combines the new and interesting DBD dye with the carbohydrate ligand mannose via a Click reaction. We demonstrate the binding to E. coli bacteria over a large concentration range and also present some special characteristics of those molecules that are of particular interest for the application as a biosensor. In particular, the mix-and-measure ability and the very good photo-stability should be highlighted here.