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  4. The Plasmodium falciparum blood stages acquire factor H family proteins to evade destruction by human complement
 
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2016
Journal Article
Title

The Plasmodium falciparum blood stages acquire factor H family proteins to evade destruction by human complement

Abstract
The acquisition of regulatory proteins is a means of blood-borne pathogens to avoid destruction by the human complement. We recently showed that the gametes of the human malaria parasite Plasmodium falciparum bind factor H (FH) from the blood meal of the mosquito vector to assure successful sexual reproduction, which takes places in the mosquito midgut. While these findings provided a first glimpse of a complex mechanism used by Plasmodium to control the host immune attack, it is hitherto not known, how the pathogenic blood stages of the malaria parasite evade destruction by the human complement. We now show that the human complement system represents a severe threat for the replicating blood stages, particularly for the reinvading merozoites, with complement factor C3b accumulating on the surfaces of the intraerythrocytic schizonts as well as of free merozoites. C3b accumulation initiates terminal complement complex formation, in consequence resulting in blood stage lysis. To inactivate C3b, the parasites bind FH as well as related proteins FHL-1 and CFHR-1 to their surface, and FH binding is trypsin-resistant. Schizonts acquire FH via two contact sites, which involve CCP modules 5 and 20. Blockage of FH-mediated protection via anti-FH antibodies results in significantly impaired blood stage replication, pointing to the plasmodial complement evasion machinery as a promising malaria vaccine target.
Author(s)
Rosa, T.F.A.
Flammersfeld, A.
Ngwa, C.J.
Kiesow, M.
Fischer, R.
Zipfel, P.F.
Skerka, C.
Pradel, G.
Journal
Cellular microbiology  
Project(s)
MALSIG
Funder
Deutsche Forschungsgemeinschaft DFG  
Deutsche Forschungsgemeinschaft DFG  
European Commission EC  
Link
Link
DOI
10.1111/cmi.12535
Additional full text version
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Language
English
Fraunhofer-Institut für Molekularbiologie und Angewandte Oekologie IME  
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