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Electrochemiluminescence (ECL) immunosensor for detection of Francisella tularensis on screen-printed gold electrode array

: Spehar-Délèze, Anna-Maria; Julich, Sandra; Gransee, Rainer; Tomaso, Herbert; Dulay, Samuel B.; O'Sullivan, Ciara K.


Analytical and bioanalytical chemistry 408 (2016), No.25, pp.7147-7153
ISSN: 1618-2642 (Print)
ISSN: 1618-2650 (Online)
Journal Article
Fraunhofer ICT-IMM ()
Electrochemilunescence; immunoassay; immunosensor; antibody fragment; biowarfare agent; scree-printed gold array; gole electrode array

An electrochemiluminescence (ECL) immunosensor for the rapid detection of the Francisella tularensis pathogen using whole antibodies or antibody fragments as capture biomolecule is described. A sandwich immunoassay was used with either lipopolysaccharide (LPS) or the whole inactivated bacterial cell (LVS) as a target, while Ru(bpy)3 2+-encapsulated silicate nanoparticles were linked to the secondary antibody and used as ECL labels. The assay was performed in a fluidic chip housed in a custom-built black box incorporating electronics, optics and fluidics. The obtained limit of detection for LPS was 0.4 ng/mL, while for the LVS it was 70 and 45 bacteria/mL when the capturing molecule was the whole antibody and the antibody F(ab) fragment, respectively.