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Protein-containing multilayer capsules by templating on mesoporous CaCO3 particles

POST- and PRE-loading approaches
: Balabushevich, Nadezhda G.; Lopez de Guerenu, Anna V.; Feoktistova, Natalia A.; Skirtach, Andre G.; Volodkin, Dimitry V.


Macromolecular bioscience 16 (2016), No.1, pp.95-105
ISSN: 1616-5187
Journal Article
Fraunhofer IZI ()
adsorption; co-synthesis; CaCO microparticles; layer-by-layer; protein encapsulation

Encapsulation of model proteins (catalase, insulin, aprotinin) into multilayer dextran sulphate/protamin capsules by templating on CaCO3 microparticles is investigated employing: (i) PRE-loading into CaCO3 particles by adsorption or co-synthesis and (ii) POST-loading into performed capsules. Protein encapsulation is governed by both its size and electrostatic interactions with the carbonate microparticles and multilayer shell. PRE-loading enables improved encapsulation compared to POST-loading (catalase content in capsules 630 and 70 mg · g−1). Bioactivity of encapsulated protein is not affected by interaction with multilayers but may be reduced at slightly alkaline pH due to CaCO3 hydrolysis. This study might help to successfully encapsulate fragile bio-macromolecules into multilayer capsules.