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Multilayer based microfluidic cell culture systems with integrated actuators for animal free substance testing

Poster presented at Bionection - Partnering Conference for Technology Transfer in Life Sciences, Leipzig, 1./2.10.2015
: Schmieder, Florian; Grünzner, Stefan; Ströbel, Joachim; Busek, Mathias; Sonntag, Frank

Poster urn:nbn:de:0011-n-3702436 (2.9 MByte PDF)
MD5 Fingerprint: 6dc4af7e3407ddefdee9b43d29dce369
Created on: 17.12.2015

2015, 1 Folie
Partnering Conference for Technology Transfer in Life Sciences <2015, Leipzig>
Poster, Electronic Publication
Fraunhofer IWS ()

The realization of complex 3D tissue cultures on a chip requires the implementation of closed circulation systems. These consist of several cell culture segments, storage chambers and micro pumps. So far the fabrication was accomplished by casting a silicone flow cell to a connector plate. This process is complicated, expensive and difficult to automate. It also limits the microfluidic system to be designed in a single layer [1-3]. // We developed a closed process chain to manufacture inexpensive microfluidic chips in an automated and cost effective way. The novel system is a multilayer design based on laser micro structured foils. The first step is to slice the microfluidic system design into individual layers so that each layer can be fabricated on a separate foil. The next step is the selection of the desired properties (e.g. hydrophilic, transparent …) for each foil, which depends on the functional requirements. In the third step these foils are laser processed on both sides, i.e. they are micro structured and functionalized. The final fourth step is laminating these individual foils into a multilayered system via thermal or plasma bonding. The multilayer technology is also applied to fabricate pneumatically actuated pumps and valves. For this purpose the design of these elements has to be adapted. // Microfluidic structures can be placed at different levels in the multilayer design, which results in greater functionality per chip area. Surface properties can be tailored by using foils with different properties (hydrophilic, hydrophobic) in combination with laser structuring and laser functionalization. This enables the implementation of new functions such as capillary stop valves and the selective colonialization of specific are as with cells. The foils can also be used to integrate thin film electrodes.