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Principles of whole-genome amplification

: Czyz, Zbigniew Tadeusz; Kirsch, Stefan; Polzer, Bernhard


Kroneis, Thomas (Ed.):
Whole genome amplification : Methods and protocols
New York: Springer Science+Business Media, 2015 (Methods in molecular biology 1347)
ISBN: 978-1-4939-2989-4 (Print)
ISBN: 978-1-4939-2990-0 (Online)
ISBN: 1-4939-2989-5
Book Article
Fraunhofer ITEM ()
whole-genome amplification; PCR-based amplification; ligation-mediated amplification; multiple displacement amplification

Modern molecular biology relies on large amounts of high-quality genomic DNA. However, in a number of clinical or biological applications this requirement cannot be met, as starting material is either limited (e.g., preimplantation genetic diagnosis (PGD) or analysis of minimal residual cancer) or of insufficient quality (e.g., formalin-fixed paraffin-embedded tissue samples or forensics). As a consequence, in order to obtain sufficient amounts of material to analyze these demanding samples by state-of-the-art modern molecular assays, genomic DNA has to be amplified. This chapter summarizes available technologies for whole-genome amplification (WGA), bridging the last 25 years from the first developments to currently applied methods. We will especially elaborate on research application, as well as inherent advantages and limitations of various WGA technologies.