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Laser microdissection of FFPE tissue areas and subsequent whole genome amplification by Ampli1™

: Czyz, Zbigniew Tadeusz; Stoecklein, Nikolas H.; Polzer, Bernhard


Kroneis, Thomas (Ed.):
Whole genome amplification : Methods and protocols
New York: Springer Science+Business Media, 2015 (Methods in molecular biology 1347)
ISBN: 978-1-4939-2989-4 (Print)
ISBN: 978-1-4939-2990-0 (Online)
ISBN: 1-4939-2989-5
Book Article
Fraunhofer ITEM ()
laser microdissection (LMD); whole-genome amplification (WGA); formalin-fixed paraffin-embedded (FFPE) tissue

Laser microdissection (LMD) and whole genome amplification (WGA) are valuable tools to isolate, purify, and genetically analyze cancer cells from tissue sections. In this chapter, we describe a workflow for microdissecting small regions of interest from cancer tissue, i.e. formalin-fixed paraffin-embedded (FFPE) and cryo-conserved specimens, and subsequent whole genome amplification by a deterministic WGA approach (Ampli1™ WGA).