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Laser microdissection of FFPE tissue areas and subsequent whole genome amplification by Ampli1™

 
: Czyz, Zbigniew Tadeusz; Stoecklein, Nikolas H.; Polzer, Bernhard

:

Kroneis, Thomas (Ed.):
Whole genome amplification : Methods and protocols
New York: Springer Science+Business Media, 2015 (Methods in molecular biology 1347)
ISBN: 978-1-4939-2989-4 (Print)
ISBN: 978-1-4939-2990-0 (Online)
ISBN: 1-4939-2989-5
pp.141-162
English
Book Article
Fraunhofer ITEM ()
laser microdissection (LMD); whole-genome amplification (WGA); formalin-fixed paraffin-embedded (FFPE) tissue

Abstract
Laser microdissection (LMD) and whole genome amplification (WGA) are valuable tools to isolate, purify, and genetically analyze cancer cells from tissue sections. In this chapter, we describe a workflow for microdissecting small regions of interest from cancer tissue, i.e. formalin-fixed paraffin-embedded (FFPE) and cryo-conserved specimens, and subsequent whole genome amplification by a deterministic WGA approach (Ampli1™ WGA).

: http://publica.fraunhofer.de/documents/N-366324.html