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Enzyme sensor with polydimethylsiloxane membrane and CMOS potentiostat for wide-range glucose measurements

: Mross, Stefan; Fürst, Peter; Pierrat, Sebastien; Zimmermann, Tom; Vogt, Holger; Kraft, Michael


IEEE Sensors Journal 15 (2015), No.12, pp.7096-7104
ISSN: 1530-437X
Journal Article
Fraunhofer IMS ()
glucose sensor; wide range measurement; high glucose concentrations; bioreactor monitoring; membrane; PDMS; cellulose acetate; CMOS potentiostat

We report on an electrochemical measurement setup comprising a glucose sensor and a CMOS potentiostat with a two-layer membrane as the first steps toward the development of an integrated in-situ sensor system for bioreactors. The potentiostat has a chip size of 2.1 mm x 2.5 mm and a linear current range from -220 nA to 240 nA with a linearity of R² = 0.9995. For wide range measurements of glucose concentrations in cell culture media, electrodes functionalized with the enzyme glucose oxidase were spin-coated with membranes made from polydimethylsiloxane (PDMS). A two-stage curing scheme of the PDMS was applied, and different membrane thicknesses and curing times were evaluated. With these membranes, glucose concentrations up to 500 mM were measured with a linear measurement range up to 200 mM. The sensors were successfully employed in the glucose monitoring of a culture of Saccharomyces cerevisiae to monitor the glucose consumption of the cells. For interference elimination, the cellulose acetate membranes were employed.