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Verfahren und Vorrichtung zur Detektion und zum Identifizieren von Biopartikeln

Identifying bioparticles comprises locating an individual particle on a substrate; irradiating a particle with a laser to form Raman spectrum; and comparing the formed Raman spectra with Raman spectra of different particles.
 
: Popp, J.; Roesch, P.; Petry, R.; Hofer, S.; Schuele, A.; Schmauz, G.; Lankers, M.; Burkhardt, H.; Ronneberger, O.; Peschke, K.

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DE 102004008762 A: 20040223
DE 2004-102004008762 A: 20040223
DE 102004008762 A1: 20050908
G01N0015
German
Patent, Electronic Publication
Fraunhofer IPA ()

Abstract
Es wird ein Verfahren zum Identifizieren von Biopartikeln bereitgestellt mit den Schritten: Lokalisierung einzelner Partikel auf einem Substrat (2) durch Abbilden wenigstens eines Bereichs des Substrats durch Bestrahlung mittels Streiflicht und Messung des von den Partikeln reflektierten Lichts; Bestrahlung eines lokalisierten Partikels mit einem Laser und Erzeugen eines Raman-Spektrums; Identifizieren des Partikels durch Vergleich des erzeugten Raman-Spektrums mit fuer eine Mehrzahl von verschiedenen Partikeln abgelegten Raman-Spektren. Es ist ferner ein Schritt des Differenzierens zwischen biotischen und abiotischen Partikeln ueber Messung des Fluoreszenzlichts der Partikel bei dem oder nach dem Schritt des Lokalisierens vorgesehen.

 

DE1004008762 A UPAB: 20051006 NOVELTY - Identifying bioparticles (I) comprises: locating an individual particle on a substrate (2) through irradiating at least an area of the substrate by means of light and measuring the light reflected by the particles; irradiating a particle with a laser and producing a localized Raman spectrum; and identifying (I) through comparison of the produced Raman spectrum with the Raman spectra produced by the majority of different particles. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is also included for a device (useful for carrying out the procedure) comprising a substrate (2), a detecting unit (3) (for locating individual particles on the substrate) and an identifying unit (4) (for identifying an individual particle by Raman spectroscopy). USE - The method is useful for identifying bioparticles (claimed) or biocontamination in various industries e.g. pharmaceuticals and food industry. The bioparticles include microorganisms such as bacteria. The method is useful in automated industries such as pharmaceutical production, food production and water supply and processing. ADVANTAGE - The identification of the biocontamination by the method and the device is simple, fast and reliable. The use of highly sensitive Raman-spectroscopy allows for specific identification of the bioparticles present in smaller quantities. The method does not require any sample preparation and culturing and the bioparticles are not destroyed during the detection and identification steps. The method is independent of growth phases and/or environmental influences. The time taken for identification is very less when compared to the conventional methods e.g. the time taken by the conventional microbiological methods for the identification takes place for about 3000 minutes, while this method requires only about 3 minutes for identification. By using this method in various automated industries e.g. food production, the cost and the risk due to product contamination is minimized.

: http://publica.fraunhofer.de/documents/N-35496.html