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Microelectrospotting as a new method for electrosynthesis of surface-imprinted polymer microarrays for protein recognition

 
: Bosserdt, Maria; Erdőssy, Júlia; Lautner, Gergely; Witt, Julia; Köhler, Katja; Gajovic-Eichelmann, Nenad; Yarman, Aysu; Wittstock, Gunther; Scheller, Frieder W.; Gyrcsányi, Róbert E.

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Biosensors & bioelectronics 73 (2015), pp.123-129
ISSN: 0956-5663
ISSN: 1873-4235
English
Journal Article
Fraunhofer IZI ()
molecularly imprinted polymers; surface imprinting; electrospotting; Ferritin; Scopoletin; electropolymerization

Abstract
Here we introduce microelectrospotting as a new approach for preparation of protein-selective molecularly imprinted polymer microarrays on bare gold SPR imaging chips. During electrospotting both the gold chip and the spotting tip are electrically connected to a potentiostat as working and counter electrodes, respectively. The spotting pin encloses the monomer-template protein cocktail that upon contacting the gold surface is in-situ electropolymerized resulting in surface confined polymer spots of ca. 500 µm diameter. By repeating this procedure at preprogrammed locations for various composition monomer-template mixtures microarrays of nanometer-thin surface-imprinted films are generated in a controlled manner. We show that the removal and rebinding kinetics of the template and various potential interferents to such microarrays can be monitored in real-time and multiplexed manner by SPR imaging. The proof of principle for microelectrospotting of electrically insulating surface-imprinted films is made by using scopoletin as monomer and ferritin as protein template. It is shown that microelectrospotting in combination with SPR imaging can offer a versatile platform for label-free and enhanced throughput optimization of the molecularly imprinted polymers for protein recognition and for their analytical application.

: http://publica.fraunhofer.de/documents/N-352026.html