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Antimalarial activity of granzyme B and its targeted delivery by a granzyme B-single-chain Fv fusion protein

: Kapelski, S.; Almeida, M. de; Fischer, R.; Barth, S.; Fendel, R.


Antimicrobial agents and chemotherapy 59 (2015), No.1, pp.669-672
ISSN: 0066-4804
ISSN: 1098-6596
Journal Article
Fraunhofer IME ()

We present here the first evidence that granzyme B acts against Plasmodium falciparum (50% inhibitory concentration [IC50], 1,590 nM; 95% confidence interval [95% CI], 1,197 to 2,112 nM). We created a novel antimalarial fusion protein consisting of granzyme B fused to a merozoite surface protein 4 (MSP4)-specific single-chain Fv protein (scFv), which targets the enzyme to infected erythrocytes, with up to an 8-fold reduction in the IC50 (176 nM; 95% CI, 154 to 202 nM). This study confirms the therapeutic efficacies of recombinant antibody-mediated antimalarial immunotherapeutics based on granzyme B.