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Microfluidic flow reactors with integrated micro-heaters and fluorescent temperature sensors for reaction monitoring

: Höra, C.; Shu, Z.; Beckert, E.; Nagl, S.; Belder, D.

Zengerle, R. ; Chemical and Biological Microsystems Society -CBMS-, San Diego/Calif.:
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Vol.2 : Freiburg, Germany, 27 - 31 October 2013
Red Hook, NY: Curran, 2014
ISBN: 978-1-63266-624-6
ISBN: 978-0-9798064-6-9
International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS) <17, 2013, Freiburg/Brsg.>
Conference Paper
Fraunhofer IOF ()

We present the combined integration of fluorescent temperature sensor layers and resistive micro-heaters in microfluidic reactor chips and their application for temperature control and monitoring during an on-chip enzymatic conversion. The production process of the chips consists of wet chemical etching, photolithography, blade coating and soft lithography. These multifunctional chips allow both temperature control and microscopic imaging of the local temperature in microfluidic flow reactors. They are thus able to determine the temperature dependency of chemical and biological reaction on the microscale. This is demonstrated by the tryptic cleavage of modified peptides yielding a fluorescent product at different temperatures.