Fraunhofer-Gesellschaft

Publica

Hier finden Sie wissenschaftliche Publikationen aus den Fraunhofer-Instituten.

Integration of fluorescent pH sensors in microfluidic free-flow isoelectric focusing platforms using automated inkjet printing

 
: Herzog, C.; Beckert, E.; Nagl, S.

Zengerle, R. ; Chemical and Biological Microsystems Society -CBMS-, San Diego/Calif.:
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Vol.2 : Freiburg, Germany, 27 - 31 October 2013
Red Hook, NY: Curran, 2014
ISBN: 978-1-63266-624-6
ISBN: 978-0-9798064-6-9
pp.1209-1211
International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS) <17, 2013, Freiburg/Brsg.>
English
Conference Paper
Fraunhofer IOF ()

Abstract
In this work, we present the integration of fluorescent pH sensor arrays, into microfluidic free-flow electrophoresis (FFE) chips. The sensor cocktail consisted a fluorescein derivate, which was linked to a hydrophilic acrylate based polymer. Optical pH sensor arrays were generated by automated inkjet printing and integrated into FFE platforms using a fabrication cycle based on multiple photopolymerisation. The functionality of microfluidic free-flow isoelectric focusing ((FFIEF) platforms was demonstrated in separations of three P503 labeled proteins via monitoring of sample and sensor fluorescence in different spectral channels. These multifunctional microanalytical devices allow nondestructive online monitoring of isoelectric points (pl).

: http://publica.fraunhofer.de/documents/N-350635.html