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  4. Electrochemical characterization of a responsive macromolecular interface on gold
 
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2015
Journal Article
Title

Electrochemical characterization of a responsive macromolecular interface on gold

Abstract
This study reports on the investigation of a thermoresponsive polymer as a thinfilm on electrodes and the influence of coupling a peptide and an antibody to thefilm. The utilized polymer from the class of poly(oligoethylene glycol)-methacrylate polymers (poly(OEGMA)) with carboxy functions containing side chains was synthesized and properly characterized in aqueous solutions. The dependence of the cloud point on the pH of the surrounding media is discussed. The responsive polymer was immobilized on gold electrodes as shown by electrochemical, quartz crystal microbalance (QCM), and atomic force microscopy (AFM) techniques. The temperature dependent behavior of the polymer covalently grafted to gold substrates is investigated using cyclic voltammetry (CV) in ferro-/ferricyanide solution. Significant changes in the slope of the temperature-dependence of the voltammetric peak current and the peak separation values clearly indicate the thermally induced conformational change on the surface. Finally, a biorecognition reaction between a short FLAG peptide (N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-LysC) covalently immobilized on the polymer interface and the corresponding IgG antibody was performed. The study shows that the responsiveness of the electrode is retained after peptide coupling and antibody binding, although the response is diminished.
Author(s)
Fandrich, A.
Buller, J.
Schäfer, D.
Wischerhoff, E.
Laschewsky, A.
Lisdat, F.
Journal
Physica status solidi. A  
DOI
10.1002/pssa.201431698
Language
English
Fraunhofer-Institut für Angewandte Polymerforschung IAP  
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