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Nanoparticles and antigen-specific T-cell therapeutics: A comprehensive study on uptake and release

 
: Zupke, Oliver; Distler, Eva; Jürchott, Anna; Paiphansiri, Umaporn; Dass, Martin; Thomas, Simone; Hartwig, Udo F.; Theobald, Matthias; Landfester, Katharina; Mailänder, Volker; Herr, Wolfgang

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Nanomedicine 10 (2015), No.7, pp.1063-1076
ISSN: 1743-5889
ISSN: 1748-6963
English
Journal Article
Fraunhofer ICT-IMM ()
cell imaging; cellular therapy; drug delivery; leukemia; nanoparticles; NP release; T lymphocytes; tumor

Abstract
Aim: T lymphocytes are used as cellular therapeutics in many disease entities including cancer. We investigated the uptake and retention of nanoparticles (NPs) by these nonphagocytic cells.
Materials & methods: Uptake, release and toxicity of various polymeric NP preparations were analyzed by flow cytometry, confocal laser scanning microscopy and transmission electron microscopy. T-cell effector functions were measured using IFN-γ-ELISPOT and 51Chromium-release assays.
Results: Amino-functionalized NPs were efficiently ingested by antigen-specific T cells without adversely influencing effector functions. NPs were stored in membrane-surrounded vesicles, with major proportions released extracellularly during 24 h.
Conclusion: Amino-functionalized polymeric NPs are efficiently taken up by human T cells and could be used to design nanocarriers for direct access and manipulation of antigen-specific T cells in vivo.

: http://publica.fraunhofer.de/documents/N-345530.html