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Optofluidic chip for surface wave-based fluorescence sensing

: Ricciardi, S.; Frascella, F.; Angelini, A.; Lamberti, A.; Munzert, P.; Boarino, L.; Rizzo, R.; Tommasi, A.; Descrovi, E.


Sensors and Actuators. B 215 (2015), pp.225-230
ISSN: 0925-4005
European Commission EC
FP7-ICT; 318035; BILOBA
Journal Article
Fraunhofer IOF ()

An optofluidic chip based on a multilayered photonic crystal structure is employed for an efficient beaming of the emitted radiation in a fluorescent microarray assay. Upon a near-field coupling of fluorescent emitters to surface modes sustained by the photonic crystal, a significant portion of radiated energy is diffracted out-of-plane towards the collection optics. The improvement in fluorescence collection is experimentally demonstrated in an antibody-antigene immunoassay. As a result, a decrease of the limit of detection for labelled antigenes by a factor of about 40 is demonstrated to occur in a robust and reproducible way, with little or no optical alignment issues.