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Multidimensional gas chromatography methods for bioanalytical research

: Wong, Y.F.; Hartmann, C.; Marriott, P.J.


Bioanalysis 6 (2014), No.18, pp.2461-2479
ISSN: 1757-6180
ISSN: 1757-6199
Journal Article
Fraunhofer IVV ()

Multidimensional gas chromatography (MDGC) methods are high-resolution volatile chemical separation techniques, and comprise classical heart-cutting MDGC and its more recent incarnation, comprehensive 2D GC. Although available for a long period, MDGC approaches are still not widely practiced in the field of bioanalysis, possibly reflecting the general preference for regular GC versus MDGC approaches. With the recent introduction of -omic' techniques that emphasize global nontargeted profiling of metabolites within living systems, it is evident that MDGC is gaining momentum as a separation tool, since it offers very high resolution. By untangling metabolites within highly complex biological matrices, and expanding the metabolic coverage, MDGC plays a frontline role in -omics' based studies. This review highlights state-of-the-art MDGC approaches, and summarizes the recent developments in bioanalytics.