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Enzymatic esterification of free fatty acids in vegetable oils utilizing different immobilized lipases

: Haar, Daniela von der; Stäbler, Andreas; Wichmann, Rolf; Schweiggert-Weisz, Ute


Biotechnology letters 37 (2015), No.1, pp.169-174
ISSN: 0141-5492 (Print)
ISSN: 1573-6776 (Online)
Journal Article
Fraunhofer IVV ()

Different immobilized lipases were screened for their ability to esterify free fatty acids (FFA) with monoacylglycerol (MAG) as acyl-group acceptor. A lipase from Rhizomucor miehei (Lipozyme RMIM) was the most suitable for lipase-catalyzed de-acidification—a promising alternative to conventional neutralization. A reduction of the FFA content to 0.6 % (w/w) was achieved by applying a substrate with an initial FFA-content of 6 % (w/w), the reaction at 50 °C for 22 h as well as the stepwise addition of a quadruple stoichiometric amount of MAG.