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A high-throughput cellulase screening system based on droplet microfluidics

 
: Ostafe, R.; Prodanovic, R.; Ung, WL.; Weitz, DA.; Fischer, R.

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Biomicrofluidics. Online journal 8 (2014), No.4, Art. 041102, 4 pp.
http://bmf.aip.org/bmf/about.jsp
ISSN: 1932-1058
English
Journal Article
Fraunhofer IME ()

Abstract
A new ultra-high-throughput screening assay for the detection of cellulase activity was developed based on microfluidic sorting. Cellulase activity is detected using a series of coupled enzymes leading to the formation of a fluorescent product that can be detected on a chip. Using this method, we have achieved up to 300-fold enrichments of the active population of cells and greater than 90% purity after just one sorting round. In addition, we proved that we can sort the cellulase-expressing cells from mixtures containing less than 1% active cells.

: http://publica.fraunhofer.de/documents/N-319680.html