Hier finden Sie wissenschaftliche Publikationen aus den Fraunhofer-Instituten.

A high-throughput cellulase screening system based on droplet microfluidics

: Ostafe, R.; Prodanovic, R.; Ung, WL.; Weitz, DA.; Fischer, R.


Biomicrofluidics. Online journal 8 (2014), No.4, Art. 041102, 4 pp.
ISSN: 1932-1058
Journal Article
Fraunhofer IME ()

A new ultra-high-throughput screening assay for the detection of cellulase activity was developed based on microfluidic sorting. Cellulase activity is detected using a series of coupled enzymes leading to the formation of a fluorescent product that can be detected on a chip. Using this method, we have achieved up to 300-fold enrichments of the active population of cells and greater than 90% purity after just one sorting round. In addition, we proved that we can sort the cellulase-expressing cells from mixtures containing less than 1% active cells.