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Fundamental improvements of the air-liquid interface technique for use in in vitro inhalation toxicology

 
: Knebel, Jan; Ritter, Detlef; Brodbeck, Carsten

ALTEX Proceedings 3 (2014), No.1, pp.148, Abstract II-16-302
ISSN: 2194-0479
World Congress on Alternatives and Animal Use in the Life Sciences <9, 2014, Prag>
English
Abstract
Fraunhofer ITEM ()

Abstract
With the ongoing restriction of animal experiments, a paradigm shift in inhalation toxicology is initiated. In the medium-term the assessment of biological effects of airborne substances (gases, aerosols and particles) will be increasingly based on results from in vitro studies too. Today the air-liquid-interface (ALI) technique represents a promising tool in combination with several biological models (cell lines, primary cells, reconstituted 3D multi cell type models up to ex vivo tissue like PCLS) for screening purposes of inhalable compounds up to mechanistic questions. Although several studies have shown the feasibility to test airborne substances in principle, there have been still major limiting factors until now to use ALI cultures in routine experimental processes, including the practicability and applicability of the method, insight into the cellular status during exposure and efficiency of particle deposition from aerosol on the cells during exposure. To overcome these limitations and to fundamentally improve the exposure step, an exposure process (P.R.I.T.®-ALI ExpoCube®) was developed allowing a use of standard multiwell plates throughout the whole process. Additionally, a possibility of online monitoring of the cellular status during the exposure by fluorescence live cell staining was realized and thermophoresis was introduced to improve the particle deposition.

: http://publica.fraunhofer.de/documents/N-315134.html