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Modul, System und Verfahren zur Analyse dreidimensionaler Strukturen

Microfluidic module for analyzing three-dimensional structures, comprises a module body, a channel for a hydrodynamic positioning of structure at constriction, and flow-moderate electrodes for enabling impedimetric measurements of structure
 
: Schmidt, Jennifer; Wegener, Joachim

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Frontpage ()

DE 102012219866 A1: 20121030
German
Patent, Electronic Publication
Fraunhofer EMFT ()

Abstract
Ein mikrofluidisches Modul zur Analyse dreidimensionaler Strukturen weist einen Modulkörper mit einer Unterseite, die für eine Positionierung auf einem Objekttisch eines Mikroskops konfiguriert ist auf. In dem Modulkörper ist ein Kanal gebildet, der einen Kanaleinlass, eine Engstelle und einen Kanalauslass aufweist, wobei der Kanal für eine hydrodynamische Positionierung der dreidimensionalen Struktur an der Engstelle beim Durchströmen des Kanals von dem Kanaleinlass zu dem Kanalauslass mit einer Flüssigkeit konfiguriert ist. Eine strömungsmäßig vor der Engstelle an den Kanal angrenzende erste Elektrode und eine strömungsmäßig nach der Engstelle an den Kanal angrenzende zweite Elektrode sind konfiguriert, um impedimetrische Messungen bezüglich der an der Engstelle hydrodynamisch positionierten dreidimensionalen Struktur zu ermöglichen. Zumindest Abschnitte des Modulkörpers sind aus einem lichtdurchlässigen Material gebildet und der Modulkörper ist konfiguriert, um eine mikroskopische Untersuchung der an der Engstelle hydrodynamisch positionierten dreidimensionalen Struktur zu ermöglichen.

 

Microfluidic module (2) comprises a module body (10, 16) having a bottom, which is configured for positioning a microscope on an object stage; a channel formed in the module body, comprising a channel inlet (20), a constriction (24) and a passage outlet, and configured for a hydrodynamic positioning of a three-dimensional structure at the constriction; and a flow-moderate first electrode and a flow-moderate second electrode, for enabling impedimetric measurements of the hydrodynamically positioned three-dimensional structure at the constriction. Microfluidic module (2) comprises a module body (10, 16) having a bottom, which is configured for positioning a microscope on an object stage; a channel formed in the module body, comprising a channel inlet (20), a constriction (24) and a passage outlet, and configured for a hydrodynamic positioning of a three-dimensional structure at the constriction during a flow with a liquid through the channel from the channel inlet to the passage outlet; and a flow-moderate first electrode configured before the constriction adjacent to the channel, and a flow-moderate second electrode configured after the constriction adjacent to the channel, for enabling impedimetric measurements of the hydrodynamically positioned three-dimensional structure at the constriction. At least portions of the module body are made of a light transmissive material, and the module body is configured to allow the microscopic examination of the hydrodynamically positioned three-dimensional structure at the constriction. Independent claims are also included for: (1) a system for analyzing three-dimensional structures comprising (a) the microfluidic module, (b) a fluid circuit, which is fluidically coupled with the channel and configured around the channel to flow through with a liquid, such that a three-dimensional structure is positioned hydrodynamically at the constriction in the channel, (c) a microscope with the object stage, arranged to make microscopic photographs of the three-dimensional structure, when the three-dimensional structure is positioned hydrodynamically at the constriction, and (d); a circuit arrangement for performing impedimetric measurements using the first- and the second electrode, when the three-dimensional structure is positioned hydrodynamically at the constriction; and (2) analyzing the three-dimensional structures using a microfluidic module, comprising positioning the microfluidic module on the object table of a microscope, flowing through the channel with a liquid, such that the three-dimensional structure is positioned hydrodynamically at the constriction in the channel, performing impedimetric measurements using the first- and the second electrode, when the three-dimensional structure is positioned hydrodynamically at the constriction, and generating the microscopic images of the three-dimensional structure, when the three-dimensional structure is hydrodynamically positioned at the constriction.

: http://publica.fraunhofer.de/documents/N-311075.html