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Verfahren zum Herstellen einer Leukozytenpräparation und Leukozytenpräparation

METHOD FOR PRODUCING A LEUKOCYTE PREPARATION, AND LEUKOCYTE PREPARATION
 
: Altrichter, Jens; Mitzner, Steffen; Lübcke, Antje; Selleng, Kathleen; Doss, Fanny

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Frontpage ()

DE 102012209673 A1: 20120608
German
Patent, Electronic Publication
Fraunhofer IZI ()

Abstract
The invention relates to a method for producing a leukocyte preparation from a leukocyte fraction. The invention further relates to a correspondingly produced or producible leukocyte preparation, and to the use thereof. The method according to the invention comprises the following method steps: a) sedimentation of the leukocyte fraction until a dividing line has formed between the sedimented erythrocytes and a leukocyte supernatant, separation of the leukocyte supernatant from the sediment, wherein the leukocyte supernatant is collected in, or remains in, a leukocyte container, b) sedimentation of the leukocytes in the leukocyte container, wherein a supernatant forms that is low in leukocytes, and removal of the supernatant low in leukocytes from the leukocyte container, c) washing of the sediment in the leukocyte container with a saline solution, wherein the sedimented cells are resuspended in the saline solution, the solution is sedimented and a resulting supernatant is removed, and d) resuspension of the sediment in the leukocyte container in a storage solution.

: http://publica.fraunhofer.de/documents/N-310952.html