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Method for the generation of a monoclonal plant cell line

 
: Kirchhoff, Janina; Schillberg, Stefan; Schiermeyer, Andreas; Schinkel, Helga; Fischer, Rainer

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Frontpage ()

WO EP2011/02785 A1: 20110607
English
Patent, Electronic Publication
Fraunhofer IME ()

Abstract
The invention provides a method for the generation of a monoclonal plant cell line from a heterologous population of plant cells, comprising the following steps: (a) provision of a heterologous population of plant cells; (b) preparation of protoplasts from said heterologous population of plant cells; (c) separation of single protoplasts by subjecting the preparation of protoplasts to flow cytometric sorting; (d) regeneration of a separated single transformed protoplast until the formation of a microcolony by co-cultivation in the presence of feeder cell material; (e) removal of the microcolony from the feeder cell material and cultivation of the microcolony until the formation of a monoclonal plant cell line.

: http://publica.fraunhofer.de/documents/N-310838.html