Fraunhofer-Gesellschaft

Publica

Hier finden Sie wissenschaftliche Publikationen aus den Fraunhofer-Instituten.

Quantification of pulmonary inflammation after segmental allergen challenge using turbo-inversion recovery-magnitude magnetic resonance imaging

 
: Vogel-Claussen, Jens; Renne, Julius; Hinrichs, Jan; Schönfeld Christian; Gutberlet, Marcel; Schaumann, Frank; Winkler, Carla; Faulenbach, Cornelia; Krug, Norbert; Wacker, Frank; Hohlfeld, Jens Michael

:

American Journal of Respiratory and Critical Care Medicine 189 (2014), No.6, pp.650-657
ISSN: 1073-449X
ISSN: 0003-0805
ISSN: 1535-4970
English
Journal Article
Fraunhofer ITEM ()
magnetic resonance imaging; segmental bronchial allergen challenge; Asthma; Bronchoscopy

Abstract
RATIONALE:
There is a need to develop novel noninvasive imaging biomarkers that help to evaluate antiinflammatory asthma treatments.
OBJECTIVES:
To investigate whether the extent of the segmental lung edema measured noninvasively using turbo-inversion recovery-magnitude magnetic resonance imaging (TIRM MRI) corresponds to the severity of the regional allergic reaction determined by the percentage of eosinophils in bronchoalveolar lavage fluid (BAL) 24 hours after segmental allergen challenge in patients with asthma compared with normal control subjects.
METHODS:
Eleven volunteers with allergic asthma and five healthy volunteers underwent segmental challenges with different allergen doses by two bronchoscopies 24 hours apart. They had lung MRI at baseline and 6 and 24 hours after segmental challenge. MRI TIRM scores were correlated with the eosinophilic response at 24 hours.
MEASUREMENTS AND MAIN RESULTS:
In patients with asthma, there were significant differences of eosinophil percentages in BAL at 24 hours from segments given standard-dose, low-dose, or no allergen (saline) (P < 0.001). Correspondingly significant differences between the TIRM score in allergen standard-dose, low-dose, and saline-treated segments were observed at 24 hours post-challenge (P < 0.001). With increasing TIRM score at 24 hours the percent eosinophils per segment 24 hours post-challenge also increased accordingly (P < 0.001). There was interobserver agreement for TIRM score grading (kappa = 0.72 for 24-h time point).
CONCLUSIONS:
The MRI-based noninvasive TIRM score is a promising biomarker for the noninvasive detection of the inflammatory response after segmental allergen challenge in patients with asthma and may serve to monitor the therapeutic effectiveness of novel antiinflammatory drugs in future human trials.

: http://publica.fraunhofer.de/documents/N-307314.html