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Kinetic modeling of rhamnolipid production by Pseudomonas aeruginosa PAO1 including cell density-dependent regulation

 
: Henkel, M.; Schmidberger, A.; Vogelbacher, Markus; Kühnert, Christian; Beuker, J.; Bernard, Thomas; Schwartz, T.; Syldatk, C.; Hausmann, R.

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Applied microbiology and biotechnology 98 (2014), No.16, pp.7013-7025
ISSN: 0171-1741
ISSN: 0175-7598
ISSN: 1432-0614
English
Journal Article
Fraunhofer IOSB ()
Rhamnolipid; biosurfactant; pseudomonas aeruginosa PAO1; process model; modeling; quorum sensing

Abstract
The production of rhamnolipid biosurfactants by Pseudomonas aeruginosa is under complex control of a quorum sensing-dependent regulatory network. Due to a lack of understanding of the kinetics applicable to the process and relevant interrelations of variables, current processes for rhamnolipid production are based on heuristic approaches. To systematically establish a knowledge-based process for rhamnolipid production, a deeper understanding of the time course and coupling of process variables is required. By combining reaction kinetics, stoichiometry, and experimental data, a process model for rhamnolipid production with P. aeruginosa PAO1 on sunflower oil was developed as a system of coupled ordinary differential equations (ODEs). In addition, cell density-based quorum sensing dynamics were included in the model. The model comprises a total of 36 parameters, 14 of which are yield coefficients and 7 of which are substrate affinity and inhibition constants. Of all 36 parameters, 30 were derived from dedicated experimental results, literature, and databases and 6 of them were used as fitting parameters. The model is able to describe data on biomass growth, substrates, and products obtained from a reference batch process and other validation scenarios. The model presented describes the time-course and interrelation of biomass, relevant substrates, and products on a process level while including a kinetic representation of cell density dependent regulatory mechanisms.

: http://publica.fraunhofer.de/documents/N-303587.html